Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification

Title: Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification (Methods in Enzymology, Volume 326) (Methods in Enzymology)
Author: Melvin I. Simon (Editor)
Publisher: Academic Press
Publication Date: 2000-10
Number Of Pages: 634

Summary:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents

Contributors to Volume 326 ix
Preface xiii
Volumes in Series xv
Section I. Historical Overview
1. The All Purpose Gene Fusion 3
Section II. Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells
2. A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli 11
3. Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions 35
4. Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study of Bacterial Gene Expression in Host Cells 47
5. IVET and RIVET: Use of Gene Fusions to Identity Bacterial Virulence Factors Specifically Induced in Host Tissues 73
6. Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotuberculosis Invasin 97
Section III. Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells
7. Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast 107
8. Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stability in Saccharomyces cerevisiae 120
9. Use of Fusions to Thymidine Kinase 134
10. In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster 146
11. Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme 159
12. Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters 165
13. Novel Methods for Chemiluminescent Detection of Reporter Enzymes 175
14. Fusions to Chloramphenicol Acetyltransferase as a Reporter 202
15. Fusions to [beta]-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells 221
Section IV. Functional Tags for Biochemical Purification
16. Purification of Proteins Using Polyhistidine Affinity Tags 245
17. Generating Fusions to Glutathione S-Transferase for Protein Studies 254
18. Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Proteins 271
19. Streptavidin-Containing Chimeric Proteins: Design and Production 305
20. Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purification 312
21. Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escherichia coli 322
22. Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Binding Peptides 340
23. The S Tag Fusion System for Protein Purification 362
24. Fusions to Self-Splicing Inteins for Protein Purification 376
25. Fusion Proteins Containing Cellulose-Binding Domains 418
26. Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags 430
27. Biotinylation of Proteins in Vivo: A Useful Post-translational Modification for Protein Analysis 440
Section V. Hybrid Proteins for Detection and Production of Antigens and Antibodies
28. Methods for Generating Multivalent and Bispecific Antibody Fragments 461
29. Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes 480
30. Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display 506
31. Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Class I Epitopes 516
32. Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell Epitopes into Antigen-Presenting Cells 527
33. Use of Anthrax Toxin Fusions to Stimulate Immune Responses 542
34. Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development 551
Author Index 571
Subject Index 601

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[ Last edited by sinapdb on 2007-1-31 at 18:16 ]